Extended Data Fig. 10: The habenulo-raphe pathway disrupts reward-conditioned responses in a value-dependent manner and does not affect unconditioned responses.
From: Nonlinear recurrent inhibition through facilitating serotonin release in the raphe
a, Confocal image depicting a representative example of LHb injection site. b, Mean licking across all small reward trials for a single mouse depicting control trials (black) or 20 Hz optogenetic stimulation trials (blue). c, Mean licking across all small reward trials for a single mouse depicting control trials (black) or 5 Hz optogenetic stimulation trials (blue). d, Mean licking across all no reward trials for a single mouse depicting control trials (black) or 20 Hz optogenetic stimulation trials (blue). e, Mean licking across all no reward trials for a single mouse depicting control trials (black) or 5 Hz optogenetic stimulation trials (blue). f-g depict post-reward optogenetic stimulation experiments. f, Mean licking across all large reward trials for a single mouse depicting control trials (black) or trials where 20 Hz optogenetic stimulation was delivered during the post-reward period (blue). g, Change in post-reward licking during optogenetic stimulation trials, compared to pre-stimulated baseline period (n = 6 mice, paired two-sided t-test, unstimulated vs stimulated reward licking). h, Correlation between mean trace licking (large reward trials) and optogenetic effect size for 20 Hz stimulation (dark blue) and 5 Hz stimulation (light blue) for trace stimulation during large reward trials. i, Correlation between effect size for 20 Hz stimulation and 5 Hz stimulation for trace stimulation during large reward trials.
