Extended Data Fig. 8: The measured lifetime of MeCP2-HT is consistent across individual mice and cell-types.

a, Example coronal section of the lifetime of MeCP2-HT after segmentation of NeuN positive nuclei. Same data as Fig. 1c-f n = 5 animals b, Example assignment of neuronal nuclei to brain regions based on the Allen CCF v3 at different levels (left 5 regions, right 50 regions). c, Protein lifetime measurements were consistent between mice as assessed by examining correlations between all possible pairs of animals (n = 5 animals, 10 pairs). For three levels of the CCF (5, 10, and 50 regions) the correlation between regions was higher than for a shuffled control. d, Two additional sections were stained in each of the five animals for oligodendrocytes (SOX10, top) and microglia (Iba1, bottom). e, MeCP2 was more abundant in the nuclei of neurons (Iba1: 0.63 ± 0.21, NeuN: 2.42 ± 0.8 SOX10: 0.84 ± 0.28 µM total dye-ligand signal; two-sided ANOVA F(2) = 27.22, p = 2e-4).Plotting mean and SD for n = 5 – individual symbols. f, Lifetimes were similar for all cell types, as confidence intervals overlapped between all cell types (median and [5th-95th percentiles] of: Iba1: 9.3 [8.4-10.1], NeuN: 8.7 [6.9-10.3], SOX10: 8.1 [7.1-9.1] days of MeCP2 lifetime). Plotted are median with 25-75 percentile as the box and 5–95 CI as the whiskers from n = 3000 bootstrap iterations.