Extended Data Fig. 5: Injection pharmacokinetics of JF dyes.

a, Design of experiment to measure dye-ligand injection pharmacokinetics. Continuous imaging was performed during carotid artery perfusion of JF₆₆₉-HTL at different rates. b, One animal, using infusion rates of 20 µl/min and 40 µl/min. c, Another animal, using 20, 40, and 80 µl/min. d, Slope ratios for all transitions in infusion rates (two animals, three transitions). If clearance was proportional to the amount of dye-ligand injected, a slope ratio of 2 is expected. The slope ratio of > 2 implies sublinear clearance or saturation of clearance mechanisms. e, 1D diffusion model shows an increase in the area of saturation (y-axis) with increasing dye-ligand injection rate (x-axis). See Supplementary Text for further details.