Fig. 5: Ventral subiculum inactivation does not affect the content of schema cells in the OFC during performance on an established problem.

a–c, Scatter plots showing the correlation coefficients of each neuron from the control (left) and GtACR2 (right) sessions. The y axes plot the correlation coefficients from unshuffled data and the x axes the mean correlation coefficients obtained after shuffling data (1,000×) to disrupt contributions of information related to the epoch (a), reward (b) or position (c). Orange, gray or black cells had actual correlation coefficients >99% of the shuffled results, indicating a significant contribution of the shuffled type of information to the correlated firing patterns. These populations, the percentage of the total of that category noted on the panels, were not affected by inactivation (χ² < 3.4; P > 0.066; d.f. = 1; χ² test). Orange denotes schema cells and gray nonschema cells. d, Venn diagrams summarizing data from a to c, showing the fraction of schema neurons recorded in control and GtACR2 sessions that were affected by the shuffling of information related to epoch (light gray), reward (light green) and position (dark gray). The sizes of circles are normalized to the total number of neurons recorded in each group and proportions in each category that overlap between categories were not affected by inactivation (χ² < 0.40; P > 0.54; d.f. = 1; χ² test). e, Food consumption across trials in the neophobia task. Lines show new food consumed per trial as a percentage of familiar food. Light green, control and deep green, GtACR2. A three-way ANOVA revealed a significant main effect of novelty (F_(1,104) = 9.11; P = 0.0032; \({{{\eta }}}_{{\rm{p}}}^{2}\) = 0.081; n = 5 trials for both groups) and a significant interaction between the novelty and group (F_(1,104) = 4.05; P = 0.047; \({{{\eta }}}_{{\rm{p}}}^{2}\) = 0.038; n = 5 trials for both groups). Further testing showed a significant difference between groups on the last three (F_(1,34) = 7.21; P = 0.01; \({{{\eta }}}_{{\rm{p}}}^{2}\) = 5.6 × 10⁻³; n = 3 trials for both groups) but not the initial two trials (F_(1,22) = 0.95; P = 0.34; \({{{\eta }}}_{{\rm{p}}}^{2}\) = 0.042; n = 3 trials for both groups). The error bars are the s.e.m.