Fig. 2: Single- and multi-bank recordings across multiple visual cortical areas.
From: Large-scale high-density brain-wide neural recording in nonhuman primates
a, Visual areas within macaque neocortex shown in a sagittal section. Inset, the estimated probe trajectory of one multi-bank recording. b, Spike waveforms of single neurons recorded across a single bank of (384) recording sites (3.84 mm) shown at their measured location on the probe surface. c, Population spike raster aligned to stimulus onset for units shown in b. d, Distribution of RFs of 202 visually responsive neurons across cortical depth in a single-bank recording. The arrows denote abrupt changes in RF progressions and putative visual area boundaries. e, Top view of c, illustrating the coverage of RFs across the contralateral visual field. f, The number of units identified by Kilosort 2.0 for each of the five probes recorded in the same location. Each probe was repeatedly used for up to 23 successive sessions. Units with firing rate >3 Hz are included. g, Spike waveforms of 3,029 single neurons recorded across 5 banks of recording sites (~19 mm) shown at their measured location on the probe surface. h, Heatmap of stimulus-evoked responses for all 2,729 visually responsive neurons. Each neuron is plotted at its corresponding cortical depth. The dashed black line denotes stimulus onset and the gray line at the top the 0.1-s duration. The gray shading on the right denotes depths where RFs fell on the LVM, HM or UVM. i, RF heat maps for 1,500 of the most superficial neurons, indicating visual field locations where stimuli evoked responses for each single neuron. The white crosshair in each map denotes the estimated horizontal and vertical meridians, with each map covering 26 (H) × 32 (V) d.v.a. RFs are arranged in a 42 (rows) × 36 (columns) array. Ntotal, total number of neurons; Nvisual, number of visually responsive neurons.
