Extended Data Fig. 5: Additional analyses of the effects on the representational maps induced by microablation.
From: Homeostasis of a representational map in the neocortex
a. Normalized correlations averaged across diagonal elements (dark green) and non-diagonal elements (light green) in the similarity matrices analogous to Fig. 1i (mean ± s.e.m. across mice, n = 9), however, applying the same filter to remove high and low category neurons (Methods) as in the analyses for the other experimental cohorts. Two-sided t-test between baseline days vs. days after ablation with FDR correction, in average of diagonal elements (Average of diagonal elements: p = 0.93, 0.93, 0.53, 0.084 for day 7, 9, 11, 15). * p < 0.01. b-d. To illustrate observed variability in the data obtained from individual mice, the thick lines show average (as in in Fig. 2e, g) and thin lines show individual normalized correlation of population vectors in diagonal part (left) and in non-diagonal part (right) from individual mice as independent samples, respectively, for sound responsive ablation cohort (b), non-sound responsive cohort (c) and control cohort (d). e. Cumulative distribution of baseline normalized correlation coefficient along diagonal elements (left) and along non-diagonal elements (right) on day 5 in the representational map for each experimental cohort (n = 69, 73, and 59 FOVs for sound responsive, non-sound responsive and control cohorts, respectively). To make sure that the distributions of normalized correlation in the representational map during baseline were comparable between the experimental cohorts, difference in these cumulative distributions was tested by Dunn’s test: Qcritical = 2.39, all Q values between groups for both diagonal and non-diagonal components in the similarity matrices, Q < Qcritical. Two-sided Mann-Whitney U test between groups with FDR correction for both the diagonal and the non-diagonal components, all p values, p > 0.5. f. Cumulative distribution of baseline normalized correlation coefficient on day 7 in the representational map. Difference in these cumulative distributions between the experimental cohorts was again tested by Dunn’s test as well as Two-sided Mann-Whitney U test. The cumulative distribution in sound responsive ablation cohort significantly relatively shifted to lower correlation than the other cohorts: For diagonal components, Qcritical = 2.39; Sound responsive ablation vs. Control: Q = 2.93; Sound responsive ablation vs. Non-responsive ablation: Q < Qcritical; Non-responsive ablation vs. Control: Q < Qcritical; Two-sided Mann-Whitney U test with FDR correction between groups (Two-color asterisks, * p < 0.05; see Supplementary Table 2.); Sound responsive ablation vs. Non-responsive ablation: p = 0.077; Non-responsive ablation vs. Control: p = 0.20; For non-diagonal components, Sound responsive ablation vs. Control: Q = 3.44; Sound responsive ablation vs. Non-responsive ablation: Q = 2.57; Non-responsive ablation vs. Control: Q < Qcritical; Two-sided Mann-Whitney U test with FDR correction between groups (* p < 0.05). g. Cumulative distribution of baseline normalized correlation coefficient during day 9–11 in the map. Dunn’s test: Qcritical = 2.39, all Q values between groups for both diagonal and non-diagonal components in the similarity matrices, Q < Qcritical. Two-sided Mann-Whitney U test between groups with FDR correction for both the diagonal and the non-diagonal components, all p values, p > 0.08. h-j. Similarity matrices from trial-averaged population response vectors across days in sound responsive ablation condition, non-sound responsive ablation condition and control, respectively. k. Simulated non-diagonal correlation of similarity matrix by experimentally derived downscaled tuning curve in individual neurons. Two-sided t-test between baseline correlation vs. simulated correlation after downscaling (mean ± s.e.m. across mice; Sound responsive cohort: p = 8.3 × 10−3; Non-sound responsive cohort: p = 0.19; Control: p = 0.27). * p < 0.05, ** p < 0.01; Two-sided t-test between simulated correlation after downscaling vs. correlation on day 7 (* p < 0.05). n = 10 mice for sound responsive ablation, n = 10 mice for non-sound responsive ablation, n = 9 mice for control. l-n. Similarity matrices across days from population response vectors after shuffling neurons across FOVs for each experimental cohort. See Supplementary Table 2. for detailed statistics.
