Fig. 6: Microablation differentially affects population responses of excitatory and inhibitory neurons.
From: Homeostasis of a representational map in the neocortex
a, An FOV in mouse auditory cortex showing broad expression of GCaMP6m and H2B::mCherry (left) and selective expression of nuclear-localized tagBFP in interneurons (right); scale bar, 100 μm. White arrows indicate neurons with mDLx-driven tagBFP expression. b, Averaged tuning curves of sound-responsive excitatory (Exc; dark red) and inhibitory (Inh; dark blue) neurons normalized to maximal response during a baseline day in the control cohort (n = 7 mice). c, Same as Fig. 3a but normalized response reliability across trials for all excitatory (left) and all inhibitory (right) neurons across days in the three experimental cohorts. Data were analyzed by two-sided t-test between baseline and postablation days with an FDR correction (top asterisks; *P < 0.05) and permutation test across groups (bottom asterisks; *P < 0.05). d, Fraction of sound-responsive excitatory (left) and inhibitory (right) neurons across cohorts. Data were analyzed by two-sided t-test with an FDR correction (postablation excitatory neurons: P > 0.30 for all cohorts; inhibitory neurons in the sound-responsive cohort: *P < 0.05). e, Same as Fig. 3d but normalized best response amplitude of responsive excitatory (left) and inhibitory (right) neurons. Data were analyzed by one-sided t-test with an FDR correction (sound-responsive cohort for days 7, 9, 11 and 15: excitatory neurons, P = 0.45, 0.045, 1.7 × 10−3 and 3.0 × 10−4; inhibitory neurons, P = 0.053, 0.043, 0.078 and 0.17; top asterisks: *P < 0.05, **P < 0.01, ***P < 0.001) and permutation test comparing ablation cohorts to the control cohort (bottom asterisks; *P < 0.05). f, Same as Fig. 4a but normalized fraction of neurons responsive on two consecutive days for excitatory (left) and inhibitory (right) neurons across cohorts. Data were analyzed by two-sided t-test comparing overlap during baseline (days 1 → 3 and 3 → 5) versus postablation (days 5 → 7, 7 → 9, 9 → 11 and 11 → 15; *P < 0.05) and by two-sided t-test with an FDR correction for group comparisons in the postablation period (right side, two-colored asterisks; *P < 0.01). Data are presented as mean ± s.e.m. across mice for b–f; sound-responsive ablation (n = 5), non-sound-responsive ablation (n = 5) and control (n = 7), except for permutation tests. Vertical yellow lines indicate the time point of ablation procedure. See Supplementary Table 1 for detailed statistics.
