Extended Data Fig. 7: Anatomical distributions of reward-relative and track-relative cells.
From: A flexible hippocampal population code for experience relative to reward
a) Example multi-plane imaging session (mouse m18, day 14) showing the mean field of view (FOV) in deep CA1 (left) and superficial CA1 (right). Cell ROIs are colored by their remapping category on this day (orange = reward-relative, blue = track-relative). Plots to the left and above each image show the kernel density estimate of the center of mass (COM) of each ROI in the ML and AP axes, respectively. Note that the FOV is shown in pixels (x = 796 pixels, y = 512 pixels) rather than microns for quantification purposes, and here the FOV is slightly truncated in the AP (x) axis due to the deadband where the laser turns around during bidirectional imaging. b) Distributions of the COM of each ROI in the ML axis, measured within each FOV, per mouse and remapping category. Deep and superficial planes of multi-plane animals are shown separately. Cell counts from all 7 switch days are combined as no discernible differences were observed across days. In (b, c), *p < 0.0038 Bonferroni-corrected significance level, **p < 0.0001, two-sided Wilcoxon rank-sum tests between RR and TR cells. Single-plane animals: m3: Z = 2.59, p = 9.51e-3, n = 427 RR, 440 TR cells; m4: Z = 0.27, p = 0.79, n = 366 RR, 955 TR cells; m7: Z = 3.22, p = 1.26e-3, n = 418 RR, 225 TR cells; m11: Z = 0.80, p = 0.42, n = 138 RR, 197 TR cells; m12: Z = 0.53, p = 0.59, n = 867 RR, 872 TR cells; m13: Z = 1.90, p = 0.058, n = 181 RR, 138 TR cells; m14: Z = 0.26, p = 0.79, n = 722 RR, 1077 TR cells; m15: Z = 0.01, p = 0.99, n = 967 RR, 1011 TR cells; m19: Z = 1.14, p = 0.25, n = 295 RR, 375 TR cells. Multi-plane animals: m17 deep: Z = 2.72, p = 6.57e-3, n = 137 RR, 240 TR cells; m18 deep: Z = 1.51, p = 0.13, n = 535 RR, 465 TR cells; m17 superficial: Z = 4.20, p = 2.86e-5, n = 141 RR, 237 TR cells; m18 superficial: Z = 2.47, p = 0.014, n = 785 RR, 795 TR cells. c) Same as (b) but in the AP axis. Single-plane animals: m3: Z = 0.40, p = 0.69; m4: Z = 0.28, p = 0.78; m7: Z = 1.19, p = 0.23; m11: Z = 0.95, p = 0.34; m12: Z = 0.47, p = 0.641; m13: Z = 0.41, p = 0.68; m14: Z = 2.03, p = 0.043; m15: Z = 0.40, p = 0.69; m19: Z = 3.31, p = 9.38e-4. Multi-plane animals: m17 deep: Z = 0.00, p = 0.998; m18 deep: Z = 0.03, p = 0.98; m17 superficial: Z = 2.85, p = 4.40e-3; m18 superficial: Z = 1.16, p = 0.25. d) Fractions of cells in each remapping category observed in the deep (black) and superficial (gray) planes of the multi-plane animals, m17 (top row) and m18 (bottom row). Right-hand columns show a bias for more cells to be detected in general in the superficial sublayer, consistent with its higher cell density. e) Fractions of cells in each remapping category shown in (d) expressed as a ratio to the fraction of all cells detected per plane, demonstrating no clear bias for RR or TR cells to either the deep or superficial sublayer. f) Estimation of depth per ROI in single-plane animals, shown for example mouse m4. Centered at the imaging FOV (left), a z-stack is taken from −100 to +100 microns around the FOV. Red lines illustrate mediolateral slices (right; z-stack side-views). Yellow lines mark the plane of the FOV. g) Estimated CA1 pyramidal layer curvature (see Methods) for example mouse m4 (left) and overlaid ROI depth estimate from day 14 (duplicated on the mean FOV, right). ROIs are color coded by estimated distance from the center of the pyramidal layer, where blue corresponds to the superficial sublayer and pink to the deep sublayer. Clipping mask (left) marks 110% of the kernel density estimate of the region containing ROIs. h) Distributions of the estimated depth of each ROI in the DV axis, per single-plane mouse and remapping category. Same n as in (b, c). *p < 0.0055 Bonferroni-corrected significance level, two-sided Wilcoxon rank-sum tests between RR and TR cells. m3: Z = 2.71, p = 6.74e-3; m4: Z = 0.44, p = 0.66; m7: Z = 1.53, p = 0.13; m11: Z = 0.11, p = 0.92; m12: Z = 3.08, p = 2.06e-3; m13: Z = 0.17, p = 0.86; m14: Z = 0.30, p = 0.77; m15: Z = 0.41, p = 0.68; m19: Z = 0.06, p = 0.95.
