Extended Data Fig. 10: Regional distribution of cortical interneuron subtypes in adult pig brain.
a, Schematic illustrating anterior and posterior coronal sections of a 1-year-old pig brain. Red boxed areas indicate cingulate cortex (CC), temporal cortex (TC) and occipital cortex (OC). CC and TC are the main destinations of Arc-derived migratory neurons, while OC is less associated with Arc-derived migratory streams. b, The cortical regions of the 1-year-old pig brain immunostained for interneuron markers. Left: PV (green)/VIP (red) double-immunostained neocortical section from the temporal cortex (TC). Right: SST (green)/CALB2 (red) double-immunostained neocortical section from the TC. The white dotted line delineates the border of cell-dense regions of the cortical layer 2. Scale bars, 100 µm. This experiment has been repeated three times. c–e. High magnification images of each cortical area in a show that VIP+ interneurons are abundant, most notably in cortical layers 2–3 of the cingulate cortex (CC) and temporal cortex (TC). Some interneurons in the adult pig TC co-express SST and CALB2 markers. Scale bars, 50 µm. f, The density of each population is measured across cortical layers. Subpopulation expressing VIP is abundant in the Arc-associated cortical regions, including CC and TC, but not OC. The data are presented as mean ± s.e.m. of counts performed on n = 2 cases in three independent experiments. g, The distribution of the density of each population across cortical layers. Whiskers extend to the minimum and maximum values of the data. Two-tailed unpaired t test. The data are presented as mean ± s.e.m. of counts performed on n = 2 cases in three independent experiments. Sample size and p values are provided as source data.
