Fig. 1: Stable differentiation competence in progenitors of GABAergic neurons.
From: Temporal control of progenitor competence shapes maturation in GABAergic neuron development in mice
a, Schematic illustrating the difference between maturation and differentiation. b, Summary of methods used to investigate the competence of progenitors located in the GE. c, UMAP plot showing single cells derived from scRNA-seq, TrackerSeq and FT datasets aligned in Monocle3 (n = 41,460 cells; n = 40 embryos). Colors correspond to dataset type. d, UMAP plot showing single cells derived from scRNA-seq, TrackerSeq and FT datasets, with each color representing a different cluster. e, UMAP plot showing scRNA-seq datasets (n = 25,297 cells; n = 20 embryos), with colors indicating various collection stages. f, UMAP plot showing single cells from ventral (GABAergic lineage) and dorsal (glutamatergic lineage) telencephalon (n = 75,431 cells), with colors indicating various collection stages. g, Pearson’s correlation plot between dorsal and ventral progenitors at different developmental stages (*P < 0.05, **P < 0.01; two-sided t-test). h, Lineplot showing relative cell number of dorsal (left) and ventral (right) postmitotic neuronal states across stages. The annotation of dorsal cell states is derived from the original publication. i, Schematic illustrating whole-cell recording for resting membrane potential. j, Boxplots showing membrane potential in cortical and GE progenitors at e13.5 and e15.5; significance was tested using two-sided, unpaired t-tests (Cortex, n = 14; GE, n = 37) with false discovery rate correction. The median and 25th and 75th percentiles are represented as middle line and border lines of boxplots, with whiskers indicating the minimum and maximum value. k, UMAP plot showing TrackerSeq barcoded cells (n = 9,938), each color representing a stage of IUE; IUE at e12.5 and e16.5; scRNA-seq after 96 h. l, UMAP plot showing cell states at the branches used for clone grouping. m, Upset plot showing clonal intersections in TrackerSeqe12.5+96h. n, Upset plot showing clonal intersections in TrackerSeqe16.5+96h. o, Barplot showing the frequency of dispersing and nondispersing clones in TrackerSeqe12.5+96h and TrackerSeqe16.5+96h. IN, interneuron; MT, mitotic; PN, projection neuron.
