Extended Data Fig. 7: Experimental perturbation of Nfib and associated phenotype.
From: Temporal control of progenitor competence shapes maturation in GABAergic neuron development in mice
a, Design of sgRNAs for tCROP-seq experiments and overview of experimental procedure. b, Design of plasmids for Nfib OE experiments and overview of experimental procedure. c, Western blot showing increased expression of exogenous NFIB in Neuro2A-cells (n=1). d, UMAP embedding of inhibitory precursors and their progenitors in Nfib/x KO. Cells are colored by clusters, which are annotated based on broad cell states and the top two marker genes; mt: mitotic; IN: interneuron precursor; iPN: inhibitory projection neuron precursor; NA: not assigned. e, Proportion change in Nfib/x KO. Cells were grouped into broad cell states, by aggregating clusters. f, Proportion change in Nfib OE. Cells were grouped into broad cell states by aggregating predicted labels. g, Number of differentially expressed (DE) genes per cluster between conditions in Nfib/x KO. Color indicates positive or negative enrichment. h, Number of DE genes per cluster between conditions in Nfib OE. Color indicates positive or negative enrichment.
