Fig. 3: Nfib regulates a shift in gene regulatory programs.
From: Temporal control of progenitor competence shapes maturation in GABAergic neuron development in mice
a, An eGRN graph displaying positive interactions between TFs active in APs. Node color indicates enrichment score by stage and node size indicates the number of direct targets per TF. Select TFs are annotated. Direct interactions originating from NFIB are highlighted. b, An eGRN subgraph highlighting downstream targets of Nfib, Tcf4 and Meis2 at e16.5. Nfib, Tcf4 and Meis2 nodes are indicated by node shape. Interactions between Nfib, Tcf4 and Meis2 are highlighted. Node color reflects the enrichment score by stage. c, Heatmap displaying signal enrichment of NFIB peaks across datasets: NFIB and H3K4me3 CUT&RUN at e16.5 GE, and scATAC-seq at e12.5 and e16.5. d, Heatmap displaying signal enrichment of e12.5- and e16.5-enriched peaks across datasets: NFIB and H3K4me3 CUT&RUN at e16.5 GE, and scATAC-seq at e12.5 and e16.5. e, Genome browser tracks of putative enhancer regions for Tcf4 and Meis2 and gene loci for Dlx2 and Dlx5, featuring NFIB CUT&RUN and scATAC-seq at e12.5 and e16.5. f, Enriched TF motifs in NFIB CUT&RUN peaks. TFs are ordered by their P value (binomial test). For each TF, the motif logo, target- and background percentage and the resulting enrichment are shown. The dendrogram on the left shows the sequence similarity of motif logos.
