Extended Data Fig. 8: EndoA1 localizes Itsn1 near the active zone.
From: Intersectin and endophilin condensates prime synaptic vesicles for release site replenishment
a, Immunofluorescence of intersectin-1 and Bassoon in EndoA WT and EndoA TKO hippocampal neurons. Scale bar 10 µm. b, Quantification of Itsn1 intensity at Bassoon puncta in EndoA WT and EndoA TKO neurons. Bars are the mean; error bars are s.e.m. Two-sided Student’s t test. *p = 0.0239. c, Immunofluorescence of intersectin-1 and Bassoon in EndoA TKO hippocampal neurons compared to EndoA TKO expressing full-length endophilin A1 (TKO + EndoA1), intersectin-1 binding mutant EndoA1 (TKO + EndoA1EKSK), or EndoA TKO cells treated with TTX (TKO + TTX). TTX (1 µm, 14–16 h) was used to silence activity in TKO cultures. Scale bar 10 µm. d, Quantification of Itsn1 intensity at Bassoon puncta in the different conditions. Bars are the mean; error bars are s.e.m. One-way ANOVA with Dunnett’s multiple comparison test. *p = 0.0194, ***p = 0.0008.
