Extended Data Fig. 10: Itsn1 WT and KO slice recordings reveal neurotransmitter release differences.
From: Intersectin and endophilin condensates prime synaptic vesicles for release site replenishment
a, Release probability in Itsn1 WT and Itsn1 KO slices measured by comparing the fEPSP slope to fiber volley amplitudes in response to stimulations of increasing mV size (20 mV, 40 mV, 60 mV, 80 mV and 100 mV). fEPSPs, field excitatory postsynaptic potentials. Dotted lines are linear regression analyses. Error bars are s.e.m. b, Plot showing paired-pulse ratio in Itsn1 WT and Itsn1 KO slices. A second pulse was separated by 20 ms, 50 ms, 500 ms and 1 s after the first pulse. The paired-pulse ratio was determined by dividing the peak of the second pulse by the initial pulse peak. Dots are the mean; error bars are s.e.m. c, Cumulative (cum.) total charge transfer (mV) measured from field excitatory postsynaptic potentials (fEPSPs) in response to stimulation; 100 AP, 20 Hz train. Transparent fill around traces is the s.e.m. d,e, Cum. total charge transfer from a, separated by the first 10 ms from each pulse (d) and the next 40 ms (e). The first 10 ms is defined as synchronous, while the next 40 ms is asynchronous. Transparent fill around traces is the s.e.m. f, Last cum. total charge transfer values measured from fEPSPs in response to a 100 AP, 20 Hz train. Bars are the mean, and error bars are s.e.m. g, Last cum. synchronous (first 10 ms of release per stimulation) charge transfer values measured from fEPSPs in response to a 100 AP, 20 Hz train. Bars are the mean, and error bars are s.e.m. h, Last cum. asynchronous (last 40 ms of release per stimulation) charge transfer values measured from fEPSPs in response to a 100 AP, 20 Hz train. Bars are the mean, and error bars are s.e.m. i, Normalized fEPSP amplitude from the first 10 pulses during the train. Transparent fill around traces is the s.e.m. j, Experimental paradigm schematic of synaptic recovery experiments in Fig. 7e. Following 100 AP, 20 Hz trains, single recovery stimulations were applied first at 100 ms after the train, then 300 ms and then with delays increasing by 200 ms every next recovery stimulation n.
