Fig. 8: A new model for release site replenishment.
From: Intersectin and endophilin condensates prime synaptic vesicles for release site replenishment
a, Schematic representation showing a new model of activity-dependent replenishment of release sites during sustained neurotransmission and short-term plasticity. The RRP consists of the docked vesicle pool and the replacement vesicle pool. The replacement vesicle pool is maintained by Itsn1 and EndoA1, while the reserve pool is maintained by Syn1. Following stimulation, the vacated release sites are rapidly replenished by replacement vesicles to enhance synaptic signaling. b, In the absence of Itsn1 condensates, the replacement pool is depleted, and replenishment fails due to lack of replacement vesicle substrate. c, With only Itsn1WEYE present, the replacement pool is also diminished and dysfunctional. d, In EndoA TKO, the replacement pool becomes smaller and is bereft of Itsn1. As a result, vesicles are not mobilized for replenishment at the release site.
