Extended Data Fig. 1: Tracing of PAM and non-PAM expansion dynamics during amyloid pathology in Cx3cr1^CreERT2R26^Confetti5xFAD⁺ animals.

Illustrative confocal images of Confetti⁺ microglia in 20-week-old Cx3cr1^CreERT2R26^Confetti5xFAD⁺ animals. Immunofluorescence for anti-GFP (Confetti⁺, green), and thioflavine-S/Thiazine Red/Methoxy-X04 (blue) are depicted together with the following markers (cyan): upper three rows: ApoE, Clec7a (higher magnification and individual channels from Fig. 2) and Axl; lower row: P2RY12 (higher magnification and individual channels from Fig. 1). Arrowheads indicate non-PAM labelled by Axl. Scale bars = 50 µm.