Fig. 6: Breathing 11% O2 starting 6 weeks after PFF injection reverses movement disorders and further loss of DA neurons in the SNpc.
From: Hypoxia ameliorates neurodegeneration and movement disorder in a mouse model of Parkinson’s disease
a, Schematic depicting the experimental groups and time course. b,c, Results of pole (b) and cage hang (c) tests in mice that had a PFF injection in the ‘PFF, Nx-Nx’ and ‘PFF, Nx-Hx’ groups. n = 12 in each group. b,c, A two-way ANOVA followed by Tukey’s correction for post-hoc comparisons was conducted. Data are presented as the mean + s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001 or ****P < 0.0001 versus day 0 in the same group, or #P < 0.05 or ###P < 0.001 versus the ‘PFF, Nx-Nx’ group at the same time point. d,e, Time in the inner zone (d) and the total distance (e) in the OFT in mice that were injected with the α-syn monomer or PFF in the ‘mon, Nx-Nx’, ‘PFF, Nx’, ‘PFF, Nx-Nx’ and ‘PFF, Nx-Hx’ groups. n = 12 in each group. d,e, A one-way ANOVA followed by Tukey’s correction for post-hoc comparisons was conducted. f, Heatmap showing the location of mice during the OFT. Each image shows the average latency of mice in each group. g, Deposition score of phosphorylated α-syn in the SNpc in mice that were treated with PFF in the ‘PFF, Nx’, ‘PFF, Nx-Nx’ and ‘PFF, Nx-Hx’ groups. n = 12 in each group. h, Representative photomicrograph of the SNpc stained for phosphorylated α-syn in mice injected with PFF in the ‘PFF, Nx’, ‘PFF, Nx-Nx’ and ‘PFF, Nx-Hx’ groups. n = 12 in each group. i, Number of TH+ neurons in the SNpc in mice treated with α-syn monomer or PFF in the ‘mon, Nx-Nx’, ‘PFF, Nx’, ‘PFF, Nx-Nx’ and ‘PFF, Nx-Hx’ groups. n = 12 in each group. j, Representative photomicrograph of the SNpc stained for TH in mice injected with α-syn monomer or PFF in the ‘mon, Nx-Nx’, ‘PFF, Nx’, ‘PFF, Nx-Nx’ and ‘PFF, Nx-Hx’ groups. n = 12 in each group. Nx, normoxia (breathing 21% O2); Hx, hypoxia (breathing 11% O2). g,i, A one-way ANOVA followed by Tukey’s correction for post-hoc comparisons was conducted. d,e,g,h, Data are presented as box plots showing the median and IQR, with the whiskers denoting the maximum and minimum values. h,j, Scale bars, 100 μm.
