Extended Data Fig. 2: RNA-seq, TMT proteomics, qPCR.
From: Hypoxia ameliorates neurodegeneration and movement disorder in a mouse model of Parkinson’s disease
(a) and (b): Venn diagrams show the numbers of shared and distinct downregulated (a) and upregulated (b) genes when comparing the transcriptomic effects of hypoxia treatment (11% O2, monomer vs 21% O2, monomer) to the effects of PFF treatment (21% O2, PFF vs 21% O2, monomer). (c) and (d): Heatmaps show the z-score scaled expression across condition for all 186 downregulated genes (c) and all 271 upregulated genes (d) in the 21% O2, PFF versus 21% O2, monomer comparison. Columns (genes) were hierarchically clustered using fastcluster.linkage (v1.2.6), with metric=euclidean and method=single. N = 12 in each group. Results of RT-qPCR for Iba1 (e), Vegfa (f), Tfrc (g), Slc16a3 (h) and Slc40a1 (i). N = 3-8. Two-way ANOVA followed by Dunnett’s correction for post-hoc comparisons was conducted for panel (e–i).
