Fig. 3: Interneuron stability across days and DNMS training.
From: Voltage imaging reveals hippocampal inhibitory dynamics shaping pyramidal memory-encoding sequences
a, Example PV cell during four DNMS trials before and after DNMS training. Expanded FOV (left). b, Firing rates per trial and odor-specific averages across the two recording sessions, plotted as in Fig. 2. c,d, Same as a,b for an SST cell. e, Average firing rates across trials for pooled PV (top) and SST cells (bottom) on any session X and following session X + 1. Cells stacked by maximum rate time bin on session X. Cells recorded for >2 sessions shown independently for each consecutive pair. White dots show significant fields. Black circles show nonsignificant firing peaks. f, Same for last naive session versus first trained session. g, Percentage cells recorded across two consecutive sessions that retained odor spiking (‘stable’), moved their firing peak into (‘inflow’) or out of the odor delivery time bins (‘outflow’). n = 7 and eight session pairs for PV and SST; *Pstable-inflow = 0.013; Pstable-outflow = 0.016; Pstable-stable = 0.049; all other P > 0.05; two-sided WT; FDR corrected. h, Mean cross-correlation of a cell’s firing rates across all trials between two sessions, as a function of distance between the sessions. Pre-Post, same between last naive versus first trained session (P > 0.05, two-sided WT for both cell groups). Small jitter added for clarity. ρS, Spearman correlation between distance of trained sessions and firing rate correlations (P > 0.05 for both cell groups). i,j, Performances of PV-Cre (i) and SST-Cre mice (j) per recording (dots) and mean ± s.e. per day (lines) (top). Naive and trained sessions indicated on top. Note that multiple training days exist between the two groups. Mean ± s.e. (middle). Percentage field cells over all PV (i) and SST cells (j) per session. Lines indicate individual mice. Mean ± s.e. odor selectivity of cells (absolute values) (bottom). Pooled distributions for naive versus trained sessions (right). For PV, from top, n = 31 versus 73 recordings, 10 versus 18 sessions, 31 versus 75 cells; ***P = 2.24 × 10−4, two-sided WT. For SST, from top, n = 35 versus 49 recordings, 10 versus 15 sessions, 36 versus 51 cells: ***P = 6.58 × 10−10, two-sided WT.
