Extended Data Fig. 2: Spiking and subthreshold properties of field cells and their correlation with locomotion and odors-OFF.

a. Average de-spiked ΔF/F traces of odor-field cells (left) and delay-field cells (right) around their field time point for individual cells (thin lines) and averaged across cells (thick). b. Average de-spiked ΔF/F traces of PV and SST odor-field and delay-field cells during the first odor delivery (gray box). Top: Odor-specific cells. Bottom: Non-specific cells. No significant differences observed at any time point in the odor (P > 0.05, WT). c. Mean firing rates across trials, speed scores and ROI sizes (number of pixels of each ROI) for PV and SST field cells (n = 58 PV, 45 SST cells) versus non-field cells (n = 49 PV, 48 SST). * P = 0.0399, ** P = 0.001, all other P > 0.05, two-sided WT. d. Mean theta amplitude, strength of theta modulation of spiking (vector length) and theta phase (measured in 1/π, 0 corresponds to theta peaks for field cells versus non-field cells, plotted as in c. P = 0.0329 for SST theta power, P = 0.014 for PV theta phase, all other P > 0.05; parametric Watson–Williams test for theta phase and two-sided WT for power and modulation. e-f. Mean firing rates of individual PV (e) and SST (f) odor-field cells (thin lines) and averaged across cells (thick) during odor delivery for trials with immobility (left) and locomotion (right) during odors. Corresponding locomotion traces shown on top row. g. Example traces from a PV cell across 4 trials, displayed as in Fig. 1c. Gray boxes: Odor cue windows in trials where the odor was not delivered. h. Top row: Firing rates from 3 PV cells across trials with alternating odors ON and OFF (scaled by maximum average rate across odor/delay bins). Second row: Same firing rates rearranged over all odor ON and OFF trials (separated by white line). Third row: Mean ± SE firing rates across odor ON and OFF trials (black and red respectively). Bottom row: Average locomotion in the same trials. i. Mean ± SE firing rates (averaged across all odor/delay bins and all corresponding trials) of each cell (left) and corresponding mean locomotion (right) in odor ON versus odor OFF trials. n = 8 cells; P = 0.0108, 0.083; paired-sample two-sided t-test. j–l. Same as G-I for SST cells. In panel l: n = 6 cells; P = 0.0184, 0.683; paired-sample two-sided t-test.