Extended Data Fig. 3: Serotonin input to the adBNST is similar in males and females.
From: Serotonergic modulation of the BNST–CeA pathway reveals sex differences in fear learning
(a) Representative image demonstrating overlap of TpH2-ChR2-EYFP neurons (green) with TpH2 immunoreactivity (far red) in the DRN and MRN, scale bar = 500 µm. (b) High magnification images of the DRN showing co-labeled cells, scale bar = 10 µm. (c) Alexa Fluor 647-conjugated cholera toxin subunit B (CTB) was injected into the right adBNST of TpH2-ChR2+ mice and expression was visualized in the raphe nuclei 10 days later (created in BioRender). Representative image from a female mouse showing TpH2-ChR2-EYFP+ neurons (green) and CTB+ neurons (far red) in the DRN and MRN. (d) High magnification of the DRN showing a co-labeled cell, scale bar = 25 µm. (e) Quantification of neurons co-labeled with TpH2-ChR2-EYFP and CTB in DRN (two-tailed unpaired t test, t(11)=0.10, p = 0.92). (f) Percent of EYFP+ cells co-labeled with CTB and EYFP in DRN (two-tailed unpaired t test, t(11)=0.73, p = 0.48). (g) Quantification of neurons co-labeled with TpH2-ChR2-EYFP and CTB in MRN (two-tailed unpaired t test, t(11)=0.51, p = 0.62). (h) Percent of EYFP+ cells co-labeled with CTB and EYFP in MRN (two-tailed unpaired t test, t(11)= 1.53, p = 0.16). n = 7 females; n = 6 males. Data are represented as mean ± SEM. DRN = dorsal raphe nucleus, MRN = median raphe nucleus.
