Extended Data Fig. 1: Stage of estrous cycle does not influence fear conditioning.
From: Serotonergic modulation of the BNST–CeA pathway reveals sex differences in fear learning
(a) Schematic of procedures indicating when vaginal swabs were taken each day. (b) Representative images of vaginal cytology of TpH2-ChR2-YFP BAC female mice. Estradiol status on the day of auditory fear conditioning did not affect (c) acquisition (two-way RM ANOVA, group, F(1,25) = 0.14, p = 0.71), (d) tone recall (two-way RM ANOVA, group, F(1,25) = 0.04, p = 0.84) or (e) recall of contextual fear memory (two-tailed unpaired t test, t(24) = 0.44, p = 0.66). n = 15 low estradiol; n = 12 high estradiol. (f) Estradiol status on the day of the tone test did not affect tone recall (two-way RM ANOVA, group, F(1,25) = 0.69, p = 0.41). n = 14 low estradiol; n = 13 high estradiol. (g) Estradiol status on the day animals were placed back in the training context (Context A) did not affect recall of contextual fear memory (two-tailed unpaired t test, t(24) = 1.56, p = 0.13). n = 13 low estradiol; n = 13 high estradiol. Data are represented as mean ± SEM. RM ANOVA = repeated measures ANOVA.
