Extended Data Fig. 6: Motor running-induced dendritic Ca²⁺ activity in control, prazosin-treated, IP₃R2KO and asGq-DDREAD.

(a–c) Time intervals between two consecutive dendritic Ca²⁺ spikes over 2 min motor training were significantly shorter in prazosin (a, control 92 time intervals, 612 time intervals), IP₃R2KO (b, control 62 time intervals, IP3R2KO 203 time intervals) and asGq-CNO (c, control 64 time intervals, asGq-CNO 160 time intervals) as compared to their respective controls. Two sided Kolmogorove-Smirnov test. P < 0.0001, = 0.0035, = 0.0076 for prazosin, IP₃R2KO and asGq-CNO compared to their respective controls. (d–h) Dendrites with repetitive Ca²⁺ activity showed significantly higher integrated Ca²⁺ activity compared to dendrites with less repetitive Ca²⁺ activity in control (d, n = 4 mice, 12 dendrites), prazosin-treated (e, n = 4 mice, 37 dendrites), IP₃R2KO (f, n = 6 mice, 25 dendrites), asGq-saline control (g, n = 3 mice, 19 dendrites) and asGq-CNO mice (h, n = 3 mice, 35 dendrites). Two sided Mann-Whitney test. P = 0.048, 0.0001, 0.0171, 0.0495, 0.0012 for control, prazosin, IP₃R2KO, asGq-saline and asGq-CNO. Boxes show the first quartile, median and third quartile of the stack data. Whiskers show minima and maxima without outliers. (i–m) The number of Ca²⁺ spikes per individual dendrite over 2 min was significantly correlated with the accumulative dendritic Ca²⁺ activity in control (i, 12 dendrites), prazosin-treated (j, 37 dendrites), IP₃R2KO (k, 25 dendrites), asGq-saline control (l, 19 dendrites) and asGq-CNO approach (m, 35 dendrites). P < 0.001, <0.001, =0.0029, =0.0011, <0.001 for control, prazosin, IP₃R2KO asGq-saline, and asGq-CNO mice. *P < 0.05, **P < 0.01, ***P < 0.001.

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