Supplementary Figure 6: Examination of base substitution and indel mutations induced by Cas9-variant-generated breaks in episomal shuttle vectors.
From: APOBEC3 induces mutations during repair of CRISPR–Cas9-generated DNA breaks
(a) Schematic diagram illustrating the procedures to determine the base substitution and indel mutations induced by Cas9 variant-generated breaks in episomal shuttle vectors. (b) Number of colonies containing mutated shuttle vectors that are induced by Cas9 variant-generated breaks. (c) Indel frequencies induced by Cas9 variant-generated breaks in episomal shuttle vectors. The means (±s.d.) were from three independent experiments. (d) Fractions at each base and distribution curves of Cas9 variant-induced indels at the cleavage site upstream and downstream 50-bp region in episomal shuttle vectors.
