Supplementary Figure 7: Distributions of indels induced by Cas9 variants.
From: APOBEC3 induces mutations during repair of CRISPR–Cas9-generated DNA breaks
(a) 293FT indel fraction at each base (indel counts at each base relative to the total indel counts in the cleavage site upstream and downstream 25-bp region, %) and the fitting curves of indel fractions in the same region. The indel fitting curves illustrating indel distribution are presented in Fig. 3c. (b) Left: Fractions at each base and distribution curves of Cas9 or D10A-induced indels in the cleavage site upstream and downstream 25-bp region for A3B cells. Right: statistical analysis of the distances from the cleavage site to curve peaks. The median, interquartile range (IQR) and 1.5 × IQR are shown. ***: P < 0.001, one-tailed Student’s t-test.
