Supplementary Figure 2: Amplification of ODN-cognate genomic region derived from HDR.
From: APOBEC3 induces mutations during repair of CRISPR–Cas9-generated DNA breaks
(a) Schematic diagrams illustrating the procedures to amplify the ODN-cognate genomic region from HDR products. (b), (c) Tag-specific genomic DNA PCR was performed using 3' end phosphorothioate modified primers, which prevent the 3' to 5' exonuclease activity of Hi-Fi DNA polymerases, to amplify the ODN-cognate genomic region from 293FT, A3B and A3Bm cells that are treated as indicated. The gels shown in (b, c) are representative of three experiments. (d), (e) Tag-specific genomic DNA PCR was performed to amplify the ODN-cognate genomic region from primary human T cells that are treated as indicated. The gels shown in (d, e) are representative of two experiments. Uncropped gel images for (b-e) are shown in Supplementary Data Set 1.
