Supplementary Figure 3: APOBEC3s expression in primary human T cells.
From: APOBEC3 induces mutations during repair of CRISPR–Cas9-generated DNA breaks
(a) Expression of endogenous APOBEC3s was up-regulated during primary human T cell activation. mRNA levels of APOBEC3 family in naive (day 0) and activated (day 3, 7 and 10) primary human CD8+ T cells were determined by RT-qPCR and normalized against TATA binding protein (TBP) mRNA levels. ND denotes non-detected. (b) Efficiency of endogenous APOBEC3s knockdown. (c) Schematic diagram illustrating that APOBEC could target both ssODN donor and the complementary genomic ssDNA for cytidine deamination, which finally cause base substitutions on Cs and Gs in genomic DNA. (a, b) The means (±s.d.) were from three independent experiments.
