Supplementary Figure 2: Normalization of RNA-DamID data.
From: RNA-DamID reveals cell-type-specific binding of roX RNAs at chromatin-entry sites
(a) RNA-DamID data is normalised by comparing enrichment of an MS2-tagged lncRNA, co-expressed with Dam-MCP over an 3xMS2 transcript co-expressed with Dam-MCP. (b) Genome browser tracks of negative control (3xMS2, Dam-MCP) and RNA-DamID (3xMS2-roX2, Dam-MCP) displayed in reads per million mapped. The negative control predominantly methylates open chromatin regions. RNA-DamID preferentially methylates roX2 targets on the X chromosome. Generating a ratio of the test over control reduces autosomal peaks. (c) roX2 RNA-DamID signal normalised to the negative control is highly correlated to roX2 RNA-DamID normalised to Dam-alone. Data is plotted as log2 normalised RNA-DamID signal enrichment over negative control.
