Supplementary Figure 1: Removal of RD does not impact on structural stability of Fes1
From: Nucleotide exchange factors Fes1 and HspBP1 mimic substrate to release misfolded proteins from Hsp70
a. CD measurements of purified Fes1 and ΔRD in phosphate buffer during heating of the samples from 20 °C to 80 °C followed by chilling to 20 °C. b. Data from A showing α-helical content before and after heating the proteins to 80°C. c. Melting temperature (Tm) of Fes1 and ΔRD determined from b at 222 nm. Data from three independent experiments are presented with their mean values and error bars representing SD.
