Supplementary Figure 10: Data processing procedures for PRC2–DiNcl40
From: Cryo-EM structures of PRC2 simultaneously engaged with two functionally distinct nucleosomes
(a) Image processing for the PRC2-DiNcl40 dataset collected at 120 kV with a 4k x 4k CCD camera. Left, representative micrograph. Middle, reference-free 2D classes to initially sort particles. Right, 3D classification resulting in a particle set of 16,333 particles used for auto-refinement. (b) Final reconstruction of the PRC2-DiNcl40 complex as top (left), front (middle) and back (right) views. (c) FSC plot indicating a final resolution of 13.3 Å according to the FSC = 0.143 criterion (Rosenthal and Henderson, J. Mol. Biol. 333, 721-745, (2003), Scheres and Chen, Nat. Methods 9, 853-854, (2012)). (d) Final EM reconstruction of PRC2-DiNcl40 with fitted atomic models for PRC2 and the DiNcl40 (for dinucleosome model generation, see methods).
