Supplementary Figure 11: Comparison of nucleosome orientations for 30-bp and 40-bp linker lengths
From: Cryo-EM structures of PRC2 simultaneously engaged with two functionally distinct nucleosomes
Atomic models of mononucleosomes (PDB ID 3LZ1) were rigid-body fitted into the EM maps of PRC2-DiNcl30 (left) and PRC2-DiNcl40 (middle). Middle left, superposition of the nucleosome DNA backbones within the PRC2-DiNcl30 (blue) and PRC2-DiNcl40 (red) complexes. Right, the distance between the DNA exit base pairs from the nucleosome was measured as a straight axis in Chimera, using corresponding terminal base pairs in the nucleosome models as anchor points. The difference between the two distances, was ~ 28 Å, compatible with an expected linker DNA length difference of ~ 33 Å, taking into account differences in the linker DNA paths due to bending.
