Supplementary Figure 2: Image processing for PRC2–AEBP2
From: Cryo-EM structures of PRC2 simultaneously engaged with two functionally distinct nucleosomes
(a) Picked particles were subjected to two rounds of 3D classification after which 102,743 particles were refined to 4.7 Å resolution overall. In order to improve the SUZ12 N-terminal region, focused classification after background subtraction was performed which helped achieve 4.6 Å overall resolution but with improved density for this sub-domain. (b) Fourier shell correlation (FSC) curve of the two independent half maps indicating an overall resolution of 4.6 Å according to the 0.143 FSC criterion (Rosenthal and Henderson, J. Mol. Biol. 333, 721-745, (2003), Scheres and Chen, Nat. Methods 9, 853-854, (2012)) (gold standard refinement). (c) EM map of PRC2 colored according to local resolution using the BLOCRES and BLOCFILT programs of the BSOFT package (Heymann and Belnap, J. Struct. Biol. 157, 3-18, (2007)).
