Supplementary Figure 5: Alignment of GPT sequences.

The sequences of human GPT, MraYAA (5CKR), and MraYCB (5JNQ) were first aligned by structural superposition, expanded to 45 GPT ortholog sequences by MAFFT, and corrected manually. Invariant positions (100% identity) are shaded red, while conserved positions (>90% identity) are shaded gray. The secondary structure of human GPT is shown, highlighting the insertion in loop E that is absent in MraY sequences (TM, transmembrane α-helix; α, α-helix; β, β-strand).