Supplementary Figure 6: Omit density of POPG lipid molecules.

Omit maps were calculated with data of the canonical hGPT (Pro129) to 3.1 Å resolution. One lipid tail of 1-palmitoyl-2-oleoylglycero-3-phosphoglycerol (POPG, magenta sticks) is found in each side fenestration. 2F o –F c omit density of POPG molecules is shown in blue mesh (contoured to 0.8 σ), while the F o –F c omit density is shown in green mesh (contoured to 3 σ). Maps were carved 1.8 Å from the POPG molecules.