Supplementary Figure 3: Composite omit electron density of hGPT. | Nature Structural & Molecular Biology

Supplementary Figure 3: Composite omit electron density of hGPT.

From: GlcNAc-1-P-transferase–tunicamycin complex structure reveals basis for inhibition of N-glycosylation

Supplementary Figure 3

2F o −F c composite omit maps were calculated from the canonical hGPT Pro129 data (3.1 Å resolution) omitting 5% of the model at a time, and contoured to 0.8 σ. a, hGPT dimer, showing ribbons for protomer A only. Omit density for protomer B is colored pink for clarity. b, View of the cytosolic cavity, showing tunicamycin and several nearby residues. c, View of the dimer interface from within the membrane, looking at protomer A from protomer B. d, View of the dimer interface from the cytosol. e, 2F o −F c composite omit map, shown for individual transmembrane helices 1 to 10 and carved 1.8 Å from the model.

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