Supplementary Figure 4: c-Abl is not required for TAp63α tetramerization, and activation of TAp63α follows a slower kinetics after cisplatin compared to doxorubicin treatment and LH treatment does not abrogate Cs induced tetramerization in oocytes
From: Oocyte DNA damage quality control requires consecutive interplay of CHK2 and CK1 to activate p63
a, TAp63α stable expressing H1299 cells were treated with increasing concentrations (5, 25 and 50 µM) of the c-Abl inhibitor imatinib 1 h prior to induction of tetramerization with Dox (10 µM, 6 h). Oligomeric state and phosphorylation induced electrophoretic mobility shift were monitored by BN-PAGE and SDS-PAGE, respectively. b, CD-1 P8 ovaries were cultured with or without 10 µM Dox or Cs for the indicated periods. Oligomeric state was monitored by BN-PAGE and phosphorylation induced electrophoretic mobility shift were SDS-PAGE, respectively. ATM total protein abundance as well as pATM levels were assessed by Western blotting. Ovaries were further subjected to 10 µM Cs in the presence of 200 mIU/ml LH.
