Supplementary Figure 5: Cryo-EM map generation and data processing flow chart of the ABCG2–MB136–Fab complex.
From: Structural basis of small-molecule inhibition of human multidrug transporter ABCG2
a, An example micrograph (drift-corrected, dose-weighted, and low-pass filtered to 20 Å) of the nanodisc-reconstituted ABCG2–MB136-Fab data set. White scale bar, 50 nm. b, Averages of 15 representative two-dimensional class averages of the final round of two-dimensional classification, sorted in decreasing order by the number of particles assigned to each class. c, The flow chart for the cryo-EM data processing and structure determination of the ABCG2-MB136-Fab complex.
