Supplementary Figure 6: Atomic model refinement and local resolution of the ABCG2–MB136–Fab complex.
From: Structural basis of small-molecule inhibition of human multidrug transporter ABCG2
a, FSC from the RELION auto-refine procedure of the unmasked half-maps (blue), the random-phase corrected half-maps (brown), the half-maps after masking (cyan), and the half-maps after masking and correction for the influence of the mask (pink). A horizontal line (blue) is drawn for the FSC = 0.143 criterion. For both the unmasked and the corrected FSC curves, their intersection with the FSC = 0.143 line is indicated by an arrow, and the resolution at this point is indicated. b, Angular distribution plot for the final reconstruction. c, Full view of the RELION local-resolution-filtered map of ABCG2-MB136-Fab colored by local resolution as calculated by ResMap with the clipping plane in the middle of the molecule. ABCG2, Fab and nanodiscs are labeled. d, Resolution distribution histogram generated in ResMap. e, Fit of one MB136 molecule into the EM density of the ABCG2-MB136-Fab structure processed with C2 symmetry. f, Fit of one MB136 molecule into the EM density of the ABCG2-MB136-Fab structure processed with C1 symmetry.
