Supplementary Figure 1: Purification of ABCG2, SEC-TS and reconstitution of ABCG2–MZ29–Fab into nanodiscs.
From: Structural basis of small-molecule inhibition of human multidrug transporter ABCG2
a, Preparative SEC profile of detergent-purified ABCG2. The fraction used for SEC-TS and nanodisc preparation is indicated with an arrow. b, SEC-TS of ABCG2 before or after the addition of specified inhibitors at 10 µM concentration and E1S at 50 µM concentration. c, Preparative SEC profile of the nanodisc-reconstituted ABCG2-MZ29-Fab complex. The fraction used for cryo-EM grid preparation and SDS-PAGE analysis is indicated by ‘1’. d, Non-reducing SDS-PAGE of the nanodisc-reconstituted ABCG2-MZ29-Fab complex shown in c.
