Supplementary Figure 3: Atomic model refinement and local resolution of the ABCG2–MZ29–Fab complex.
From: Structural basis of small-molecule inhibition of human multidrug transporter ABCG2
a, FSC from the RELION auto-refine procedure of the unmasked half-maps (blue), the random-phase corrected half-maps (brown), the half-maps after masking (cyan), and the half-maps after masking and correction for the influence of the mask (pink). A horizontal line (blue) is drawn for the FSC = 0.143 criterion. For both the unmasked and the corrected FSC curves, their intersection with the FSC = 0.143 line is indicated by an arrow, and the resolution at this point is indicated. b, FSC curve of the final 3.1 Å refined model versus the map it was refined against (FSCfull, black line). FSC curve of the final model with introduced shifts (mean value of 0.3 Å) refined against the first of two independent half-maps (half-map 1) (to which it was refined against; FSCwork red line) or the same refined model versus the second independent half-map (to which is was not refined; FSChalf2, green line). c, Full view of the RELION local-resolution-filtered map of ABCG2-MZ29-Fab colored by local resolution as calculated by ResMap with the clipping plane in the middle of the molecule. ABCG2, Fab and nanodiscs are labeled. d, Resolution distribution histogram generated in ResMap. e, Angular distribution plot for the final reconstruction.
