Supplementary Figure 7: MALDI MS of FUS LC. The mass of purified recombinant LC is in red.
From: Atomic structures of FUS LC domain segments reveal bases for reversible amyloid fibril formation
After incubation with DNA-PK (20 ng per μg LC) at 30 °C for 1 hour, the mass of LC increases indicating phosphorylation of LC (magenta). Subsequently, the phosphorylated LC was incubated with CIAP (10 U per 30 μg phosphorylated LC) at 30 °C for 30 min. The recovery of LC mass indicates dephosphorylation (blue).
