Supplementary Figure 5: RNAi is dispensable for the assembly of heterochromatin islands at 18 °C.
From: Iron homeostasis regulates facultative heterochromatin assembly in adaptive genome control
(a) Genome-wide H3K9me distribution at 18 °C in wild-type, ago1Δ, and ccr4Δ cells was determined by ChIP-chip and is plotted on all three chromosomes of S. pombe. New 18 °C facultative heterochromatin peaks are indicated. Wild-type ChIP-chip data is also presented in Fig. 2a and ccr4Δ ChIP-chip data is also presented in Fig. 6a. (b) H3K9me enrichments at 18 °C in wild-type and ago1Δ cells were determined by ChIP-qPCR. H3K9me fold enrichments at SPAPB1A11.02, SPBC428.10, and SPBC1289.14 relative to the control leu1 gene are shown as the mean +s.d. (n = 3 independent experiments). (c) H3K9me enrichments at 18 °C in wild-type, ccr4Δ, and red1Δ cells were determined by ChIP-qPCR. H3K9me fold enrichments at SPAPB1A11.02, SPBC428.10, and SPBC1289.14 relative to the control leu1 gene are shown as the mean +s.d. (n = 3 independent experiments). Wild-type ChIP-qPCR data is also presented in Supplementary Fig. 5b.
