Supplementary Figure 7: Iron affects gene regulation and the assembly of facultative heterochromatin islands at low temperature.
From: Iron homeostasis regulates facultative heterochromatin assembly in adaptive genome control
(a) Heat map of fold change values relative to wild-type 30 °C cells for up-regulated transcripts in indicated cultures grown at 18 °C. Clusters are grouped according to the analysis presented in Fig. 1f. (b) H3K9me enrichments in wild-type cells untreated or treated with 250µM Dip and grown at 18 °C for 72 hours (upper) or 24 hours (lower). H3K9me fold enrichments at indicated loci were determined by ChIP-qPCR and are shown relative to the control leu1 gene. Enrichments for the 72 hour treatments are shown as the mean +s.d. (n = 3 independent experiments). (c) H3K9me enrichments at 18 °C in ccr4Δ fep1-1 cells untreated or treated with 250µM Dip were determined by ChIP-qPCR. H3K9me fold enrichments at indicated loci are shown relative to the control leu1 gene.
