Supplementary Figure 1: Supporting data for in vitro tau aggregation screen.

a) Addition of Hsc70 to tau prior to aggregation induction leads to dose-dependent effects on aggregation. 0N4R tau^WT incubated with indicated concentrations of Hsc70 or Hsc70_NBD was induced to aggregate with the addition of heparin. The aggregation kinetic parameters (lag time, elongation rate constant, and amplitude) calculated from the corresponding ThT aggregation curves are plotted (mean ± SD, triplicates, representative of 3 independent experiments). b) Calculated elongation rate constant for tau variants tested in the in vitro aggregation screen (mean ± SD, triplicates). c and d) Replicate experiments demonstrating reproducibility of lag time effects. c) 18 chaperones were assayed for effects on 0N4R tau^WT aggregation for two independent experiments. The fold change in lag time due to addition of chaperone was plotted for each experiment on the x and y axes, respectively (mean ± SD, triplicates for each experiment). The calculated values for the Spearman’s rank correlation are shown. d) Hsc70 was assayed against the entire panel of 0N4R tau variants in triplicate within the same plate for 3 independent experiments. The fold change in lag time due to addition of full-length Hsc70 was plotted for each replicate paradigm on the x and y axes, respectively (mean ± SD). The calculated values for the Spearman’s rank correlation are shown.