Supplementary Figure 2: Data analysis of additional calculated kinetic parameters from tau aggregation screen.

a) Heatmap representing the effect of individual chaperones on the elongation rate constant parameter of aggregation for each tau variant. For each chaperone-tau combination, the log₂ fold change in elongation rate of tau when aggregated in the absence or presence of an equimolar concentration of chaperone was plotted. Red and blue represent chaperone–dependent decreases or increases in aggregation elongation rate constant, respectively. Grey = not tested. b) Dose-dependent effects of chaperone screen hits on tau aggregation lag time. Fold change in lag time of 0N4R tau^WT (10 μM) in the presence of multiple chaperone concentrations are plotted (mean ± SD, triplicates). c) Comparison of DnaJA1 and DnaJA2 effects on tau aggregation kinetics. 0N4R tau^WT (7.5 µM) was aggregated in the presence of an equimolar concentration of DnaJA1 or DnaJA2. The ThT fluorescence for each reaction is plotted (mean ± SD from triplicate wells is plotted, representative of 2 independent experiments). d) Heatmap of absolute values for lag time kinetic parameter for 0N4R tau^WT and ΔK280 in the presence of chaperones. The calculated lag time (h) is displayed according to the indicated color scale for each chaperone-tau combination (1:1 stoichiometry). The lag time value (h) for each tau variant without chaperone is included.