Supplementary Figure 4: Comparison of ligand-binding sites for the peptide and compound in the AT2R structure.
From: Crystal structure of the human angiotensin II type 2 receptor bound to an angiotensin II analog
a, Overall structures are shown as AT2R bound to [Sar1, Ile8]-AngII in green (this study) and AT2R (PDB ID: 5UNF) bound to compound 1 in gray. [Sar1, Ile8]-AngII is shown as a licorice model with a yellow backbone, and compound 1 is shown as a licorice model with a light blue backbone. b, Comparison of ECL2 of AT2R with (green) or without (gray) Fab4A03. c,d, Residues surrounding [Sar1, Ile8]-AngII and compound 1 (C1) in AT2R are shown in gray. Ligands are shown as licorice models, with the carbon atoms of [Sar1, Ile8]-AngII in yellow and C1 in light blue. AT2R is shown with the same orientation of transmembrane helices for ArgECL2. ArgECL2 and Lys2155.42 are residues critical for binding of both ligands (labeled in red). Thr1784.60 is a critical residue for C1 binding (labeled in blue).
