Supplementary Figure 6: N-terminal protein ubiquitination is not affected by proteasomal inhibition.
From: UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
a, Prevalence of amino acids at the second position of N-terminally ubiquitinated proteins in Hep2 (top) and Jurkat (bottom) cells. b, Comparison of the N-terminally ubiquitinated proteins identified by the StUbEx PLUS approach in U2OS cells (blue) (data extracted from J. Proteome Res. 17, 296, 2018) with those identified in the current UbiSite study (red). c, log10 intensities of N-terminally ubiquitinated peptides in untreated cells or upon proteasomal inhibition with bortezomib (BOR) or b-AP15 (AP15). Peptides quantified in all three replicates of at least one treatment are shown.
