Supplementary Figure 8: Comparison of binding activities of the ZZ domains of p300 and CBP.
From: The ZZ domain of p300 mediates specificity of the adjacent HAT domain for histone H3
a, Superimposed 1H,15N HSQC spectra of the same length (51-residue) constructs of p300-ZZ (top) and CBP-ZZ (bottom) collected upon titration with H3 (1–12) peptide. b, Superimposed 1H,15N HSQC spectra of the same length (46-residue) constructs of p300-ZZ (top) and CBP-ZZ (bottom) collected upon titration with H3 (1–12) peptide. c, Overlays of 1H,15N HSQC spectra of p300-ZZ (left) and CBP-ZZ (right) recorded upon titration with SUMO1. No CSPs were observed in either experiment, indicating that the p300/CBP ZZ domains do not interact with SUMO1. d, Structural overlay of p300-ZZ without H3 and apo CBP-ZZ (PDB 1TOT) suggests that the Ala1-binding site in CBP is partially occluded.
