Supplementary Figure 3: 5-HT2BR–lisuride structure and OBP mutant L3627.35 surface expression.
From: Structural determinants of 5-HT2B receptor activation and biased agonism
a, 5-HT2BR–lisuride structure Fo – Fc omit map of ligand (left), 2Fo – Fc regular map of ligand (middle) and binding pocket residues (right). b, Fo – Fc omit density map of residues T1403.37 and L3627.35 in the structure of lisuride-bound 5-HT2BR. c, Alignment of the structures for the 5-HT2BR–LSD and 5-HT2BR–lisuride complexes reveals similar positioning of the indole N(1)-H with respect to TM5. d, Surface expression measured by ELISA reveals similar expression levels of L3627.35 mutants as for wild-type 5-HT2BR. Data represent means ± s.e.m. from quadruplicate replicates from two independent experiments (n = 2).
