Supplementary Figure 4: Single-particle cryo-EM analysis of the AtOSCA1.1 structure.

a,b, Side view (a) and bottom view (b) of local resolution estimation of the final sharpened AtOSCA1.1 cryo-EM density map. c, FSC curves of the model versus half map1 (black line), half map2 (green), and full map (red). d,e, EM density segments (blue mesh) of the 11 transmembrane helices (M0–M10) (d) and representative CTD residues (e) are superimposed on the model in a stick representation.